Is It Alive Practical Part 2

(continued from Is It Alive Practical Part 1)

PART 2

Criteria of living things tested: Requires water & nutrition (food)

Procedure:

• Label each separate sector of the petri dish A, B, C, D and E.
• Fold the facial cotton and line each sector with cotton.
• Pour the content of each tube in the respective sector that you have labeled. (You may need to rinse off all the substance with another 3ml of water.)
• Add more water to make sure that the cotton is thoroughly wet.
• Tape the sides of the petri dish and bring back to your class to continue with the observations.
• Write your observation every day for the next 3 days using the same table format.

Table 3

Substance	Changes Observed			Inference/ Evidence of Life
	After 10 min	After 24h	After 36h
A	Looks wet (from water)	Unchanged.	Unchanged.	Unaffected by water --> not alive
B	Has mixed with the water to form sludge. Bad odour.	Unchanged. Many small droplets found above it.	More condensation found above it.	Many small droplets + water vapour --> signs of respiration. --> alive
C	Grew larger. Appears to have absorbed water.	Grew larger.	Unchanged.	Can grow. But may just be absorbent. --> alive?

Information gathered from groupmates:

D: Grew green shoots and leaves.
E: Unchanged.
EXTRA: When E was soaked in a saline solution for 48h, it hatched into sea monkeys!